The cross-dressing Labour-backing entertainer”s outlay was revealed in the first transparency report by voting watchdog the Electoral Commission on spending in the referendum campaign.
It detailed more than £4.5million of expenditure by 48 individuals, campaigners and groups in the run-up to the June 23 vote, largely those who spent between £10,000 and £250,000.
Details of spending by larger players including the Vote Leave and Britain Stronger In Europe officially designated lead Leave and Remain campaigns will be released next year.
Mr Izzard submitted receipts totalling £36,229.22 including £22,000 on transport and food, £12,699 on referendum “material” and £715 on media.
It was not immediately clear if it was his own cash or donations from others but he is reportedly a multi-millionaire thanks to his long showbusiness career.
The biggest single campaign declaration revealed by the Electoral Commission was £676,016 by fashion student Darren Grimes, who ran the social media-driven BeLeave campaign, with strong backing from the official Vote Leave campaign.
On the Remain side, the biggest spenders revealed were the We Are Europe group – described as a “collective of friends, artists, campaigners, creatives and doers” – who spent £326,445 trying to mobilise support among young people for staying in the EU.
Trade unions including Unite and Usdaw weighed in with spending for Remain of over £100,000 each, while the RMT train drivers” union declared £45,000 of spending on the winning Leave side.
Pub chain JD Wetherspoon was a prominent Leave backer – although it still attracted custom from Mr Izzard during the campaign – and recorded campaign spending worth £94,586.
Its chairman Tim Martin today waded back into the fray by accusing a “cabal” of politicians and elite groups of trying to overturn the referendum result.
In his firm”s magazine he said most people had accepted the result but some were trying to scupper or dilute it.
Mr Martin wrote: “The fascinating question is why these highly educated people are so intent on remaining part of an undemocratic and economically chaotic organisation.
“The sad reality is that the current battle for democracy has echoes of similar battles in previous centuries.
“Certain sections of society today feel strongly that it”s better for power to reside in highly educated elites, closely connected to big business, major universities and influential politicians.
“The evidence of history is that undemocratic organisations, like the EU, eventually make a hash of it, as we”re now seeing in southern Europe.”
Express.co.uk has contacted a representative for Eddie Izzard for comment.